Journal: bioRxiv

Article Title: Perfusion Bioreactor Culture Incorporating Mechanical Confinement Enhances Mesenchymal Stem Cell Extracellular Vesicle Production and Wound Healing Potential

doi: 10.1101/2025.08.12.669872

Figure Lengend Snippet: A) Timeline of experiment setup and injection schedule. Mice received injections of 1.1×10 10 EVs or an equivalent volume of PBS on days 0, 3, 6, and 9. B) Representative photos of splinted wounds receiving each treatment over 14 days (n=4-6). C) Percent wound closure based on wound area over 14 days (n=4-6). D) Fold change of M1 markers CD86, iNOS, and TNF11 and E) M2 markers IL-10, CD206, and Arg1 in harvested tissue from mice receiving flask and bioreactor EV injections relative to tissue from mice receiving PBS, as quantified by qPCR. Data represents 4 biological replicates analyzed with 3 technical replicates each per treatment group (n=4). F) Length of regenerating epithelium from H&E staining as a percentage of the length of the wound bed (n=4-6). G) Collagen deposition from Masson’s Trichrome staining quantified as a percentage of the total tissue volume (n=4-6). G) Granulation tissue area from H&E staining (n=4-6). I) CD31 fluorescence intensity normalized to DAPI over multiple fields of view and representative confocal microscopy images taken at 10X magnification (n=4-5). All values expressed as mean +/- standard error of the mean (ns – no significance; * p<0.05; ** p<0.01).

Article Snippet: Resulting cDNA was analyzed in a human MSC exosome miRNA qPCR array (GeneCopoeia; QM0460B6) per the manufacturer’s protocol.

Techniques: Injection, Staining, Fluorescence, Confocal Microscopy

Journal: bioRxiv

Article Title: Perfusion Bioreactor Culture Incorporating Mechanical Confinement Enhances Mesenchymal Stem Cell Extracellular Vesicle Production and Wound Healing Potential

doi: 10.1101/2025.08.12.669872

Figure Lengend Snippet: A) Heat map of the miRNA content of 5 µm static and 5 µm bioreactor EVs represented as the log2(fold change) relative to flask EVs (n≥2). B) Volcano plot comparing the miRNA within static and flask EVs, with the horizontal line at 1.3 (p = 0.05) representing statistical significance (n≥2). C) Volcano plot comparing the miRNA within bioreactor and flask EVs, with the horizontal line at 1.3 (p = 0.05) representing statistical significance. The labeled points indicate those in common between both static and bioreactor EVs (n≥2). All data represents the average of at least 2 independent experiments.

Article Snippet: Resulting cDNA was analyzed in a human MSC exosome miRNA qPCR array (GeneCopoeia; QM0460B6) per the manufacturer’s protocol.

Techniques: Labeling

Journal: Acta Pharmaceutica Sinica. B

Article Title: Avenanthramide A potentiates Bim-mediated antineoplastic properties of 5-fluorouracil via targeting KDM4C/ MIR17HG /GSK-3 β negative feedback loop in colorectal cancer

doi: 10.1016/j.apsb.2024.07.018

Figure Lengend Snippet: AVN A suppresses the miR-17-92 cluster to derepress Bim level. (A, B) HCT116 (A) and HCT-8 (B) cells were treated with 5-FU, AVN A, or combination for 24 h. Western blot was utilized for the levels of Bim, Mcl-1, Bcl-xL, Bcl-2, PUMA, and Bik. (C) CRC cells were exposed to 5-FU treatment alone and the combination and cultured with 1 μg/mL of actinomycin D for the indicated time. The mRNA stability of Bim was assessed using qPCR assay. (D) HCT-8 cells were treated with 5-FU and AVN A alone or together for 24 h and applied to qPCR analysis by miRNA array. Volcano plot demonstrates differentially expressed miRNAs via the combination of P values (cutoff = 0.05) and relative fold change (cutoff = 1.5). The downregulated miRNAs are shown in blue circles, whereas upregulated miRNAs are represented in red circles. (E) Following treatment with AVN A for 24 h, miR-17-92 cluster levels in CRC cells were analyzed by qPCR. (F) Heatmap of miR-17-92 cluster in the TCGA CRC database. (G) The differentially expressed miRNAs in 537 CRC samples and 9 adjacent normal tissues derived from TCGA are visualized in a volcano plot. The blue and red colors indicate low and high expression (|log2(fold change) | > 1, FDR <0.05), respectively. (H) The expression of MIR17HG in COAD based on individual cancer stage in TCGA CRC database. (I) Expression of Bim in CRC cells which transduced with two different si- MIR17HG (50 nmol/L) and then exposed to 5-FU for 24 h was detected by Western blot. (J, K) si- MIR17HG (50 nmol/L) was transferred into HCT116 (J) and HCT-8 (K) cells and then exposed to 5-FU for 24 h, and MTT assay was utilized to assess the relative cell viability. Data in (C)‒(E), (J), (K) are shown as mean ± SD, n = 3; ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.

Article Snippet: The miProfile TM human colorectal cancer miRNA array (GeneCopoeia, MD, USA) was applied for the characterization of AVN A-mediated miRNA expression.

Techniques: Western Blot, Cell Culture, Derivative Assay, Expressing, Transduction, MTT Assay

Journal: Scientific Reports

Article Title: α-Melanocyte-stimulating hormone prevents glutamate excitotoxicity in developing chicken retina via MC4R-mediated down-regulation of microRNA-194

doi: 10.1038/srep15812

Figure Lengend Snippet: The relative expression levels of MC1R, MC4R, and MC5R in the age-matched embryonic chicken retinas ( A,C,E ) and retinal explants ( B,D,F ) were measured by qPCR and shown by bar graphs. The red line connects the mean relative expression levels of the target gene at each time point, illustrating the dynamic gene expression patterns. n = 3–8/time point.

Article Snippet: Total RNA was extracted from the pooled samples by the TRIzol Reagent (Life Technologies, Grand Island, NY, USA), reverse transcribed using an All-in-One miRNA qRT-PCR Detection Kit (GeneCopoeia, Rockville, MD, USA), and subjected to a miProfileTM human toxicology-related miRNA qPCR array (GeneCopoeia, Rockville, MD, USA).

Techniques: Expressing, Gene Expression

Journal: Scientific Reports

Article Title: α-Melanocyte-stimulating hormone prevents glutamate excitotoxicity in developing chicken retina via MC4R-mediated down-regulation of microRNA-194

doi: 10.1038/srep15812

Figure Lengend Snippet: A miR array revealed that the expression levels of 12 miRs, out of the 84 miRs examined, were up-regulated more than 5-fold in the glutamate-treated explants over the α-MSH + glutamate-treated counterparts at 48 h post stimulation, n = 5/group ( A ). The relative expression levels of miR-194, the miR exhibiting the most dramatic change in the miR array, were confirmed in a separate experiment by qPCR, n = 5–10/group ( B ). ** p < 0.01.

Article Snippet: Total RNA was extracted from the pooled samples by the TRIzol Reagent (Life Technologies, Grand Island, NY, USA), reverse transcribed using an All-in-One miRNA qRT-PCR Detection Kit (GeneCopoeia, Rockville, MD, USA), and subjected to a miProfileTM human toxicology-related miRNA qPCR array (GeneCopoeia, Rockville, MD, USA).

Techniques: Expressing